Cytokeratin(Pan) Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesK5; DDD; EBS2; KRT5A; KRT5
ProductOrderC
Clone#7H8
Entrez GenelD3852
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of Cytokeratin 5 expressed in E. Coli.

Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
Sequence
N
Catalog#: 20335
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Images
Immunohistochemical analysis
Figure 1: Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma (A),normal hepatocyte (B), colon adenocacinoma, normal stomach tissue (D), showing cytoplasmic and membrane localization using CK mouse mAb with DAB staining.
Figure 1: Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma (A),normal hepatocyte (B), colon adenocacinoma, normal stomach tissue (D), showing cytoplasmic and membrane localization using CK mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 2: Confocal Immunofluorescence analysis of methanol-fixed Eca-109 cells using Cytokeratin (Pan) mouse mAb (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.
Figure 2: Confocal Immunofluorescence analysis of methanol-fixed Eca-109 cells using Cytokeratin (Pan) mouse mAb (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.
Product Overview
Description

Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors.

References (references)
References (references)1. Vet Rec. 2006, Dec 16, 159(25): 839-43.
2. J Cell Biochem. 2007, Apr 15, 100(6): 1406-14.