Cytokeratin(Pan) Primary Antibody

Item Information

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Price

Description

Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors.

Product Overview

Entrez GenelD

3852

Aliases

K5; DDD; EBS2; KRT5A; KRT5

Clone#

7H8

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of Cytokeratin 5 expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1. Vet Rec. 2006, Dec 16, 159(25): 839-43.
2. J Cell Biochem. 2007, Apr 15, 100(6): 1406-14.

Product Image

Immunohistochemical analysis

Figure 1: Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma (A),normal hepatocyte (B), colon adenocacinoma, normal stomach tissue (D), showing cytoplasmic and membrane localization using CK mouse mAb with DAB staining.

Immunofluorescence analysis

Figure 2: Confocal Immunofluorescence analysis of methanol-fixed Eca-109 cells using Cytokeratin (Pan) mouse mAb (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.

For Research Use Only. Not for use in diagnostic procedures.