Cytokeratin 8 Primary Antibody

Item Information

Catalog #

Size

Price

Description

Cytokeratin 8, also known as CK8, KRT8, K8. Entrez Protein NP_002264. It is a member of the type II keratin family.Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells.It typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.

Product Overview

Entrez GenelD

3856

Aliases

CK8; CYK8; K2C8; KRT8

Clone#

8A5D12

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human Cytokeratin (aa391-483) expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1. Cancer Genet Cytogenet. 2007 Oct 15;178(2):94-103.
2. Cancer Lett. 2008 Jul 8;265(2):188-96.

Product Image

Western Blot

Figure 1: Western blot analysis using CK8 mouse mAb against A549 (1), Hela (2), MCF-7 (3), A431 (4) and HepG2 (5) cell lysate.

Immunohistochemical analysis

Figure 2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma (A), lung cancer (B) and ovarian cancer tissue (C), showing membrane and cytoplasmic localization with DAB staining using CK8 mouse mAb.

Immunohistochemical analysis

Figure 3: Immunohistochemical analysis of paraffin-embedded human Kidney tissues using CK8 mouse mAb

Immunofluorescence analysis

Figure 4: Confocal Immunofluorescence analysis of methanol-fixed ECA109 cells (left) and HepG2 cells (right) using CK8 mouse mAb (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.

For Research Use Only. Not for use in diagnostic procedures.