CTNNB1 Primary Antibody

Item Information

Catalog #

Size

Price

Description

The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants.

Product Overview

Entrez GenelD

1499

Aliases

CTNNB; MRD19; armadillo

Clone#

5B6E9

Host / Isotype

Mouse / Mouse IgG1

Immunogen

Purified recombinant fragment of human CTNNB1 (AA: 632-781) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200-1/1000

FCM (Flow Cytometry)

1/200-1/400

ELISA

1/10000

References

1.Anticancer Res. 2016 Apr;36(4):1599-604. 2.Int J Clin Exp Pathol. 2015 Nov 1;8(11):14989-94.

Product Image

ELISA

Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

WESTERN BLOT

Figure 2: Western blot analysis using CTNNB1 mAb against human CTNNB1 (AA: 632-781) recombinant protein. (Expected MW is 36 kDa)

WESTERN BLOT

Figure 4: Western blot analysis using CTNNB1 mouse mAb against Hela (1), HepG2 (2),NIH3T3 (3),MCF-7 (4), C6 (5),COS-7 (6),K562 (7),Jurkat (8), A549 (9),SH-SY5Y (10),BEL-7402 (11), HEK293 (12), and HEK293-6e (13) cell lysate.

FLOW CYTOMETRY