CTNNB1 Primary Antibody
The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants.
Purified antibody in PBS with 0.05% sodium azide
4°C; -20°C for long term storage
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using CTNNB1 mAb against human CTNNB1 (AA: 1-100) recombinant protein. (Expected MW is 37.1 kDa)
Figure 3:Western blot analysis using CTNNB1 mAb against HEK293 (1) and CTNNB1 (AA: 1-100)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Western blot analysis using CTNNB1 mouse mAb against Hela (1), SH-SY5Y (2), NIH/3T3 (3), and HEK293(4) cell lysate.
Figure 5:Flow cytometric analysis of Hela cells using CTNNB1 mouse mAb (green) and negative control (red).
Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CTNNB1 mouse mAb with DAB staining.
Figure 7:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using CTNNB1 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.