CTNNB1 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesCTNNB; FLJ25606; FLJ37923; DKFZp686D02253; CTNNB1
ProductOrderC
Clone#4D5
Entrez GenelD1499
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human CTNNB1 expressed in E. Coli.
MW85kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
682-782
Catalog#: 20412
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Images
Western Blot
Figure 1: Western blot analysis using CTNNB1 mouse mAb against CTNNB1-hIgGFc transfected HEK293 cell lysate.
Figure 1: Western blot analysis using CTNNB1 mouse mAb against CTNNB1-hIgGFc transfected HEK293 cell lysate.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded human Placenta tissues using CTNNB1 mouse mAb
Figure 2: Immunohistochemical analysis of paraffin-embedded human Placenta tissues using CTNNB1 mouse mAb
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of A549 (left) and SK-BR-3 (right) cells using CTNNB1 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Figure 3: Immunofluorescence analysis of A549 (left) and SK-BR-3 (right) cells using CTNNB1 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric
Figure 4: Flow cytometric analysis of A549 cells using CTNNB1 mouse mAb (green) and negative control (purple).
Figure 4: Flow cytometric analysis of A549 cells using CTNNB1 mouse mAb (green) and negative control (purple).
Product Overview
Description

The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. The distinct peripheral cytosolic proteins, alpha, beta and gamma catenin (102, 94 and 86 kDa) are found in many tissues and bind to the conserved cytoplasmic tail domain of the cell adhesion cadherins. Catenins link E cadherin to other integral membrane or cytoplasmic proteins and are modulated by Wnt1 proto oncogene. The central core region of beta catenin is involved in mediation of cadherin catenin complex interaction with EGFR. Beta-Catenin-mediated signalling is involved at several stages of vertebrate neural development.

References (references)
References (references)1. Cancer Gennet Cytogenet. 2008. 187(1):12-8
2. Hepatobiliary Pancreat Dis Int. 2008. 7(5):490-6
3. J Biol Chem. 1995. 270(10):5549-55
4. EMBO J. 1998. 17(5):137-84