CTNNB1 Primary Antibody

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Description

The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. The distinct peripheral cytosolic proteins, alpha, beta and gamma catenin (102, 94 and 86 kDa) are found in many tissues and bind to the conserved cytoplasmic tail domain of the cell adhesion cadherins. Catenins link E cadherin to other integral membrane or cytoplasmic proteins and are modulated by Wnt1 proto oncogene. The central core region of beta catenin is involved in mediation of cadherin catenin complex interaction with EGFR. Beta-Catenin-mediated signalling is involved at several stages of vertebrate neural development.

Product Overview
Entrez GenelD
1499
Aliases
CTNNB; FLJ25606; FLJ37923; DKFZp686D02253; CTNNB1
Clone#
4D5
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CTNNB1 expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Cancer Gennet Cytogenet. 2008. 187(1):12-8
2. Hepatobiliary Pancreat Dis Int. 2008. 7(5):490-6
3. J Biol Chem. 1995. 270(10):5549-55
4. EMBO J. 1998. 17(5):137-84
Product Image
Western Blot
Figure 1: Western blot analysis using CTNNB1 mouse mAb against CTNNB1-hIgGFc transfected HEK293 cell lysate.
Figure 1: Western blot analysis using CTNNB1 mouse mAb against CTNNB1-hIgGFc transfected HEK293 cell lysate.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded human Placenta tissues using CTNNB1 mouse mAb
Figure 2: Immunohistochemical analysis of paraffin-embedded human Placenta tissues using CTNNB1 mouse mAb
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of A549 (left) and SK-BR-3 (right) cells using CTNNB1 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Figure 3: Immunofluorescence analysis of A549 (left) and SK-BR-3 (right) cells using CTNNB1 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric
Figure 4: Flow cytometric analysis of A549 cells using CTNNB1 mouse mAb (green) and negative control (purple).
Figure 4: Flow cytometric analysis of A549 cells using CTNNB1 mouse mAb (green) and negative control (purple).
For Research Use Only. Not for use in diagnostic procedures.