CSK Primary Antibody
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Description
Carboxy-terminal Src kinase (Csk) is a ubiquitously expressed nonreceptor tyrosine kinase that negatively regulates the Src family kinases (SFK) by phosphorylation of the SFK carboxy-terminal tyrosine. Phosphorylated carboxy-terminal tyrosine binds to the SH2 domain of SFK intramolecularly and leads to folding and inactivation of the SFK . This Csk-catalyzed SFK tyrosine phosphorylation is highly specific and exclusive. The SFK carboxy-terminal tyrosine is the only known physiological substrate of Csk .Tissue specificity: Expressed in lung and macrophages.
Product Overview
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
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Western Blot
Figure 1: Western blot analysis using CSK mouse mAb against NIH/3T3 (1)?Hela (2)?COS7 (3), Jurkat (4), Raw246.7 (5), A549 (6), HL-60 (7) and PC-12 (8) cell lysate.
Immunofluorescence analysis
Figure 2: Immunofluorescence analysis of U251 cells using CSK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric
Figure 3: Flow cytometric analysis of HL-60 cells using CSK mouse mAb (green) and negative control (purple).
For Research Use Only. Not for use in diagnostic procedures.