CSK Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesMGC117393; CSK
ProductOrderC
Clone#5F3
Entrez GenelD1445
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human CSK expressed in E. Coli.
MW50kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman, Mouse, Monkey, Rat
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
300-450
Catalog#: 30117
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Images
Western Blot
Figure 1: Western blot analysis using CSK mouse mAb against NIH/3T3 (1)?Hela (2)?COS7 (3), Jurkat (4), Raw246.7 (5), A549 (6), HL-60 (7) and PC-12 (8) cell lysate.
Figure 1: Western blot analysis using CSK mouse mAb against NIH/3T3 (1)?Hela (2)?COS7 (3), Jurkat (4), Raw246.7 (5), A549 (6), HL-60 (7) and PC-12 (8) cell lysate.
Immunofluorescence analysis
Figure 2: Immunofluorescence analysis of U251 cells using CSK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 2: Immunofluorescence analysis of U251 cells using CSK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric
Figure 3: Flow cytometric analysis of HL-60 cells using CSK mouse mAb (green) and negative control (purple).
Figure 3: Flow cytometric analysis of HL-60 cells using CSK mouse mAb (green) and negative control (purple).
Product Overview
Description

Carboxy-terminal Src kinase (Csk) is a ubiquitously expressed nonreceptor tyrosine kinase that negatively regulates the Src family kinases (SFK) by phosphorylation of the SFK carboxy-terminal tyrosine. Phosphorylated carboxy-terminal tyrosine binds to the SH2 domain of SFK intramolecularly and leads to folding and inactivation of the SFK . This Csk-catalyzed SFK tyrosine phosphorylation is highly specific and exclusive. The SFK carboxy-terminal tyrosine is the only known physiological substrate of Csk .Tissue specificity: Expressed in lung and macrophages.

References (references)
References (references)1. Nat Genet. 2009 Jun;41(6):677-87.
2. Leuk Res. 2009 Sep;33(9):e168-9.
3. J Hypertens. 2011 Jan;29(1):62-9.