CSK Primary Antibody

Item Information

Catalog #

Size

Price

Description

Carboxy-terminal Src kinase (Csk) is a ubiquitously expressed nonreceptor tyrosine kinase that negatively regulates the Src family kinases (SFK) by phosphorylation of the SFK carboxy-terminal tyrosine. Phosphorylated carboxy-terminal tyrosine binds to the SH2 domain of SFK intramolecularly and leads to folding and inactivation of the SFK . This Csk-catalyzed SFK tyrosine phosphorylation is highly specific and exclusive. The SFK carboxy-terminal tyrosine is the only known physiological substrate of Csk .Tissue specificity: Expressed in lung and macrophages.

Product Overview

Entrez GenelD

1445

Aliases

MGC117393; CSK

Clone#

5F3

Host / Isotype

Mouse / IgG1

Species Reactivity

Human, Mouse, Monkey, Rat

Immunogen

Purified recombinant fragment of human CSK expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1. Nat Genet. 2009 Jun;41(6):677-87.
2. Leuk Res. 2009 Sep;33(9):e168-9.
3. J Hypertens. 2011 Jan;29(1):62-9.

Product Image

Western Blot

Figure 1: Western blot analysis using CSK mouse mAb against NIH/3T3 (1)?Hela (2)?COS7 (3), Jurkat (4), Raw246.7 (5), A549 (6), HL-60 (7) and PC-12 (8) cell lysate.

Immunofluorescence analysis

Figure 2: Immunofluorescence analysis of U251 cells using CSK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow cytometric