COTL1 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesCLP
ProductOrderC
Clone#5C8E3
Entrez GenelD23406
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human COTL1 expressed in E. Coli.
MW16kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
full(1-142)
Catalog#: 30322
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Images
Western Blot
Figure 1: Western blot analysis using COTL1 mAb against human COTL1 (AA: 1-142) recombinant protein. (Expected MW is 16 kDa)
Figure 1: Western blot analysis using COTL1 mAb against human COTL1 (AA: 1-142) recombinant protein. (Expected MW is 16 kDa)
Western Blot
Figure 2: Western blot analysis using COTL1 mAb against HEK293 (1) and COTL1 (AA: 1-142)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using COTL1 mAb against HEK293 (1) and COTL1 (AA: 1-142)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 3: Flow cytometric analysis of Hela cells using COTL1 mouse mAb (green) and negative control (red).
Figure 3: Flow cytometric analysis of Hela cells using COTL1 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

This gene encodes one of the numerous actin-binding proteins which regulate the actin cytoskeleton. This protein binds F-actin, and also interacts with 5-lipoxygenase, which is the first committed enzyme in leukotriene biosynthesis. Although this gene has been reported to map to chromosome 17 in the Smith-Magenis syndrome region, the best alignments for this gene are to chromosome 16. The Smith-Magenis syndrome region is the site of two related pseudogenes.

References (references)
References (references)1. Biochem J. 2009 Dec 14;425(1):265-74.
2. Exp Mol Med. 2009 May 31;41(5):354-61.