COTL1 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes one of the numerous actin-binding proteins which regulate the actin cytoskeleton. This protein binds F-actin, and also interacts with 5-lipoxygenase, which is the first committed enzyme in leukotriene biosynthesis. Although this gene has been reported to map to chromosome 17 in the Smith-Magenis syndrome region, the best alignments for this gene are to chromosome 16. The Smith-Magenis syndrome region is the site of two related pseudogenes.
Product Overview
Entrez GenelD
23406
Aliases
CLP
Clone#
5C8E3
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human COTL1 expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Biochem J. 2009 Dec 14;425(1):265-74.
2. Exp Mol Med. 2009 May 31;41(5):354-61.
2. Exp Mol Med. 2009 May 31;41(5):354-61.
Product Image
Western Blot
Figure 1: Western blot analysis using COTL1 mAb against human COTL1 (AA: 1-142) recombinant protein. (Expected MW is 16 kDa)
Western Blot
Figure 2: Western blot analysis using COTL1 mAb against HEK293 (1) and COTL1 (AA: 1-142)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 3: Flow cytometric analysis of Hela cells using COTL1 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.