COL3A1 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.
Product Overview
Entrez GenelD
1281
Aliases
EDS4A; EDSVASC; PMGEDSV
Clone#
8C1G11
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human COL3A1 (AA: 24-153) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4?; -20? for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.PLoS One. 2018 Jan 18;13(1):e0191220. 2.Hum Pathol. 2016 Nov;57:176-181.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using COL3A1 mAb against human COL3A1 (AA: 24-153) recombinant protein. (Expected MW is 39.2 kDa)
Western Blot
Figure 3:Western blot analysis using COL3A1 mAb against HEK293 (1) and COL3A1 (AA: 24-153)-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometric
Figure 4:Flow cytometric analysis of Hela cells using COL3A1 mouse mAb (green) and negative control (red).
Immunohistochemical Analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using COL3A1 mouse mAb with DAB staining.
Immunohistochemical Analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using COL3A1 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.