COL2A1 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene.
Product Overview
Entrez GenelD
1280
Aliases
AOM; ANFH; SEDC; STL1; COL11A3
Clone#
7F2A11
Host / Isotype
Mouse / Mouse IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human COL2A1 (AA: 1222-1487) expressed in mammalian.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,J Clin Pediatr Dent. 2020 Sep 1;44(5):364-372.
2,Eur J Med Genet. 2020 Dec;63(12):104059.
2,Eur J Med Genet. 2020 Dec;63(12):104059.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using COL2A1 mAb against human COL2A1 (AA: 1222-1487) recombinant protein. (Expected MW is 60 kDa)
Immunofluorescence analysis
Figure 3:Flow cytometric analysis of Hela cells using COL2A1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 4:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using COL2A1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using COL2A1 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.