COL1A2 Primary Antibody
This gene encodes the pro-alpha2 chain of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in this gene, however, tend to be less severe than mutations in the gene for the alpha1 chain of type I collagen (COL1A1) reflecting the different role of alpha2 chains in matrix integrity. Three transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/31830ea_382.jpg?v=1666704649)
![Figure 2:Western blot analysis using COL1A2 mAb against human COL1A2 (AA: 23-79) recombinant protein. (Expected MW is 31.4 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/31830rwb_383.jpg?v=1666704649)
![Figure 3:Western blot analysis using COL1A2 mAb against HEK293 (1) and COL1A2 (AA: 23-79)-hIgGFc transfected HEK293 (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/31830twb_384.jpg?v=1666704649)
![Figure 4:Immunofluorescence analysis of Hela cells using COL1A2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/31830icc_386.jpg?v=1666704649)
![Figure 5:Flow cytometric analysis of SW480 cells using COL1A2 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/31830fcm_385.jpg?v=1666704649)