CNN3 Primary Antibody

Item Information

Catalog #

Size

Price

Description

This gene encodes a protein with a markedly acidic C terminus; the basic N-terminus is highly homologous to the N-terminus of a related gene, CNN1. Members of the CNN gene family all contain similar tandemly repeated motifs. This encoded protein is associated with the cytoskeleton but is not involved in contraction.

Product Overview

Entrez GenelD

1266

Clone#

5E4E4

Host / Isotype

Mouse / Mouse IgG1

Immunogen

Purified recombinant fragment of human CNN3 (AA: 26-130) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4?; -20? for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/500 - 1/2000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.PLoS One. 2014 Jul 22;9(7):e103216. 2.Arch Dermatol Res. 2013 Sep;305(7):571-84.

Product Image

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using CNN3 mAb against human CNN3 (AA: 26-130) recombinant protein. (Expected MW is 37.6 kDa)

Western Blot

Figure 3:Western blot analysis using CNN3 mAb against HEK293 (1) and CNN3 (AA: 26-130)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using CNN3 mouse mAb against Hela (1), U251 (2), and HEK293 (3) cell lysate.

Immunofluorescence analysis

Figure 5:Immunofluorescence analysis of Hela cells using CNN3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow Cytometric