CLDN6 Primary Antibody

Item Information

Catalog #

Size

Price

Description

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. This gene encodes a component of tight junction strands, which is a member of the claudin family. The protein is an integral membrane protein and is one of the entry cofactors for hepatitis C virus. The gene methylation may be involved in esophageal tumorigenesis. This gene is adjacent to another family member CLDN9 on chromosome 16.[provided by RefSeq, Aug 2010]

Product Overview

Entrez GenelD

9074

Aliases

CLDN6

Clone#

8G5D2

Host / Isotype

Mouse / Mouse IgG1

Immunogen

Purified recombinant fragment of human CLDN6 (AA: extra(29-81) and extra(138-160)) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

FCM (Flow Cytometry)

1/200-1/400

ELISA

1/10000

References

1,Oncol Rep. 2017 Aug;38(2):875-885. 2,Biotechnol J. 2018 Mar;13(3):e1700345.

Product Image

ELISA

Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

WESTERN BLOT

Figure 2: Western blot analysis using CLDN6 mAb against human CLDN6 (AA: extra(29-81) and extra(138-160)) recombinant protein. (Expected MW is 26 kDa)

WESTERN BLOT

Figure 3: Western blot analysis using CLDN6 mAb against HEK293-6e (1) and CLDN6 (AA: extra(29-81) and extra(138-160))-hIgGFc transfected HEK293-6e (2) cell lysate.

FLOW CYTOMETRY