CK5 Primary Antibody
|Aliases||KRT5; K5; DDD; DDD1; EBS2; KRT5A|
|Formulation||Purified antibody in PBS with 0.05% sodium azide|
|Immunogen||Purified recombinant fragment of human CK5 (AA: 258-357) expressed in E. Coli.|
|Shipping Information||This product will ship in a box containing blue ice at a temperature of 4°C. Learn More|
|Species Reactivity||Human, Mouse, Monkey|
|ICC (Immunocytochemistry)||1/200 - 1/1000|
|IHC_P(Immunohistochemistry)||1/200 - 1/1000|
|FCM (Flow Cytometry)||1/200 - 1/400|
|WB (Western Blot)||1/500 - 1/2000|
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 2:Western blot analysis using CK5 mAb against human CK5 (AA: 258-357) recombinant protein. (Expected MW is 33.1 kDa)
Figure 3:Western blot analysis using CK5 mAb against HEK293 (1) and CK5 (AA: 258-357)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Western blot analysis using CK5 mouse mAb against A431 (1), 3T3-L1 (2), COS7 (3), MCF-7 (4), SK-Br-3 (5), Hela (6), Lncap (7), and HepG2 (8) cell lysate.
Figure 5:Immunofluorescence analysis of MCF-7 cells using CK5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 6:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using CK5 mouse mAb with DAB staining.
Figure 7:Flow cytometric analysis of HeLa cells using CK5 mouse mAb (green) and negative control (red).
|Description||The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.|
|References (references)||1.Horm Cancer. 2013 Feb;4(1):36-49. |
2.Breast Cancer Res Treat. 2011 Jul;128(1):45-55.