CK5 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesKRT5; K5; DDD; DDD1; EBS2; KRT5A
ProductOrderC
Clone#3D6D4
Entrez GenelD3852
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human CK5 (AA: 258-357) expressed in E. Coli.
MW62.4kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman, Mouse, Monkey
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
258-357
Catalog#: 30892
Inquire
Contact us to order
Images
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using CK5 mAb against human CK5 (AA: 258-357) recombinant protein. (Expected MW is 33.1 kDa)
Figure 2:Western blot analysis using CK5 mAb against human CK5 (AA: 258-357) recombinant protein. (Expected MW is 33.1 kDa)
Western Blot
Figure 3:Western blot analysis using CK5 mAb against HEK293 (1) and CK5 (AA: 258-357)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using CK5 mAb against HEK293 (1) and CK5 (AA: 258-357)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using CK5 mouse mAb against A431 (1), 3T3-L1 (2), COS7 (3), MCF-7 (4), SK-Br-3 (5), Hela (6), Lncap (7), and HepG2 (8) cell lysate.
Figure 4:Western blot analysis using CK5 mouse mAb against A431 (1), 3T3-L1 (2), COS7 (3), MCF-7 (4), SK-Br-3 (5), Hela (6), Lncap (7), and HepG2 (8) cell lysate.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of MCF-7 cells using CK5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Immunofluorescence analysis of MCF-7 cells using CK5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using CK5 mouse mAb with DAB staining.
Figure 6:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using CK5 mouse mAb with DAB staining.
Flow cytometric
Figure 7:Flow cytometric analysis of HeLa cells using CK5 mouse mAb (green) and negative control (red).
Figure 7:Flow cytometric analysis of HeLa cells using CK5 mouse mAb (green) and negative control (red).
Product Overview
DescriptionThe protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.
References (references)
References (references)1.Horm Cancer. 2013 Feb;4(1):36-49.
2.Breast Cancer Res Treat. 2011 Jul;128(1):45-55.