CK5 Primary Antibody

Item Information

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Size

Price

Description

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

Product Overview

Entrez GenelD

3852

Aliases

KRT5; K5; DDD; DDD1; EBS2; KRT5A

Clone#

2C8E4

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human CK5 (AA: 258-357) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.Horm Cancer. 2013 Feb;4(1):36-49.
2.Breast Cancer Res Treat. 2011 Jul;128(1):45-55.

Product Image

Elisa

Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Western Blot

Figure 2:Western blot analysis using CK5 mAb against human CK5 (AA: 258-357) recombinant protein. (Expected MW is 33.1 kDa)

Western Blot

Figure 3:Western blot analysis using CK5 mAb against HEK293 (1) and CK5 (AA: 258-357)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using CK5 mouse mAb against A431 (1), MCF-7 (2), SK-Br-3 (3), Hela (4), Lncap (5), and HepG2 (6) cell lysate.

Immunofluorescence analysis

Figure 5:Immunofluorescence analysis of MCF-7 cells using CK5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric