CHRNA6 Primary Antibody
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Description
This gene encodes an alpha subunit of neuronal nicotinic acetylcholine receptors. These receptors consist of five subunits and function as ion channels involved in neurotransmission. The encoded protein is a subunit of neuronal nicotinic acetylcholine receptors that mediate dopaminergic neurotransmission and are activated by acetylcholine and exogenous nicotine. Alternatively spliced transcript variants have been observed for this gene. Single nucleotide polymorphisms in this gene have been associated with both nicotine and alcohol dependence.
Product Overview
Immunogen
Purified recombinant fragment of human CHRNA6 (AA: 26-239) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CHRNA6 mAb against human CHRNA6 (AA: 26-239) recombinant protein. (Expected MW is 51.2 kDa)
Western Blot
Figure 3:Western blot analysis using CHRNA6 mAb against HEK293 (1) and CHRNA6 (AA: 26-239)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of Hela cells using CHRNA6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 5:Flow cytometric analysis of SH-SY5Y cells using CHRNA6 mouse mAb (green) and negative control (red).
Flow cytometric
Figure 6:Flow cytometric analysis of SK-N-SH cells using CHRNA6 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CHRNA6 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.