CHRNA3 Primary Antibody

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Description

This locus encodes a member of the nicotinic acetylcholine receptor family of proteins. Members of this family of proteins form pentameric complexes comprised of both alpha and beta subunits. This locus encodes an alpha-type subunit, as it contains characteristic adjacent cysteine residues. The encoded protein is a ligand-gated ion channel that likely plays a role in neurotransmission. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer. Alternatively spliced transcript variants have been described.

Product Overview

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4°C; -20°C for long term storage

Product Image

Elisa

Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using CHRNA3 mAb against human CHRNA3 (AA: 32-240) recombinant protein. (Expected MW is 50.6 kDa)

Western Blot

Figure 3:Western blot analysis using CHRNA3 mAb against HEK293 (1) and CHRNA3 (AA: 32-240)-hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis

Figure 4:Immunofluorescence analysis of Hela cells using CHRNA3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Immunofluorescence analysis

Figure 5:Immunofluorescence analysis of SMMC-7721 cells using CHRNA3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric