CHRM3 Primary Antibody
Item Information
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Size
Price
Description
The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 3 controls smooth muscle contraction and its stimulation causes secretion of glandular tissue. Alternative promoter use and alternative splicing results in multiple transcript variants that have different tissue specificities.
Product Overview
Entrez GenelD
1131
Aliases
HM3; PBS; EGBRS
Clone#
4A6B7
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CHRM3 (AA: extra mix) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Clin Cancer Res. 2015 Oct 15;21(20):4676-85.
2.J Thorac Oncol. 2014 Feb;9(2):170-8.
2.J Thorac Oncol. 2014 Feb;9(2):170-8.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CHRM3 mAb against human CHRM3 (AA: extra mix) recombinant protein. (Expected MW is 37.9 kDa)
Western Blot
Figure 3:Western blot analysis using CHRM3 mAb against HEK293 (1) and CHRM3 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of Jurkat cells using CHRM3 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.