CEBPA Primary Antibody

Item Information

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Price

Description

The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, the encoded protein can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells.

Product Overview

Entrez GenelD

1050

Aliases

CEBP; C/EBP-alpha

Clone#

5B7

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Synthesized peptide of human CEBPA (AA: C-RKSRDKAKRNVETKV).

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

Br J Cancer. 2010 Jul 13;103(2):275-84.
Cell Res. 2010 Apr;20(4):470-9.

Product Image

Western Blot

Figure 1: Western blot analysis using CEBPA mouse mAb against Jurkat (1), k562 (2), and HepG2 (3) cell lysate.

Immunohistochemical analysis

Figure 2: Immunohistochemical analysis of paraffin-embedded rectum tissues using CEBPA mouse mAb with DAB staining.

Immunofluorescence analysis

Figure 3: Immunofluorescence analysis of HeLa cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunofluorescence analysis

Figure 4: Immunofluorescence analysis of HeLa cells using CEBPA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow cytometric