CEBPA Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, the encoded protein can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells.
Product Overview
Entrez GenelD
1050
Aliases
CEBP; C/EBP-alpha
Clone#
4E10
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CEBPA expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
Haematologica. 2009 Aug;94(8):1075-84.
Br J Cancer. 2009 Aug 18;101(4):738-40.
Br J Cancer. 2009 Aug 18;101(4):738-40.
Product Image
Western Blot
Figure 1: Western blot analysis using CEBPA mAb against human CEBPA (AA: 139-204) recombinant protein. (Expected MW is 32.7 kDa)
Western Blot
Figure 2: Western blot analysis using CEBPA mouse mAb against THP-1 (1)cell lysate.
Flow cytometric
Figure 3: Flow cytometric analysis of HeLa cells using CEBPA mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.