CEA Primary Antibody

Item Information

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Size

Price

Description

Carcino Embryonic Antigen (CEA) is synthesised during development in the fetal gut, and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. Antibodies to CEA are useful in identifying the origin of various metastatic adenocarcinomas and in distinguishing pulmonary adenocarcinomas (60 to 70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). The carcinoembryonic antigen (CEA) is a member of a large family of glycoproteins and a useful tumor marker for adenocarcinoma. Tissue specificity: Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.

Product Overview

Entrez GenelD

1048

Aliases

CEA; CD66e; DKFZp781M2392; CEACAM5

Clone#

3G12

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human CEA expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1. Scand J Clin Lab Invest. 2008;68(8):703-13.
2. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2008 Apr;24(4):370-2. Chinese.

Product Image

Western Blot

Figure 1: Western blot analysis using CEA mAb against HEK293 (1) and CEA(AA: 460-600)-hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis

Figure 2: Immunofluorescence analysis of PANC-1 cells using CEA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

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