Mouse Monoclonal Antibody to CDK4

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Description

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.

Product Overview
Entrez GenelD
1019
Aliases
CMM3; PSK-J3
Clone#
4G9F1
Host / Isotype
Mouse / IgG2a
Immunogen
Purified recombinant fragment of human CDK4 (AA: 77-303) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Cancer Res. 2019 Oct 15;79(20):5245-5259. 2,J Dermatol. 2019 May;46(5):444-448
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CDK4 mAb against human CDK4 (AA: 77-303) recombinant protein. (Expected MW is 51.6 kDa)
Figure 2:Western blot analysis using CDK4 mAb against human CDK4 (AA: 77-303) recombinant protein. (Expected MW is 51.6 kDa)
Western Blot
Figure 3:Western blot analysis using CDK4 mAb against HEK293-6e (1) and CDK4 (AA: 77-303)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using CDK4 mAb against HEK293-6e (1) and CDK4 (AA: 77-303)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using CDK4 mouse mAb against COS-7 (1), and NIH/3T3 (2) cell lysate.
Figure 4:Western blot analysis using CDK4 mouse mAb against COS-7 (1), and NIH/3T3 (2) cell lysate.
Flow cytometric analysis
Figure 5:Flow cytometric analysis of Hela cells using CDK4 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of Hela cells using CDK4 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 6:Flow cytometric analysis of HepG2 cells using CDK4 mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of HepG2 cells using CDK4 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 7:Flow cytometric analysis of Raji cells using CDK4 mouse mAb (green) and negative control (red).
Figure 7:Flow cytometric analysis of Raji cells using CDK4 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 8:Flow cytometric analysis of THP-1 cells using CDK4 mouse mAb (green) and negative control (red).
Figure 8:Flow cytometric analysis of THP-1 cells using CDK4 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 9:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CDK4 mouse mAb with DAB staining.
Figure 9:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CDK4 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 10:Immunohistochemical analysis of paraffin-embedded kidney cancer tissues using CDK4 mouse mAb with DAB staining.
Figure 10:Immunohistochemical analysis of paraffin-embedded kidney cancer tissues using CDK4 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.

Catalog#: 32132

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