CD99 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. There is a related pseudogene located immediately adjacent to this locus.
Product Overview
Entrez GenelD
4267
Aliases
MIC2; HBA71; MIC2X; MIC2Y; MSK5X
Clone#
1B10E12
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human CD99 (AA: 1-185) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200-1/1000
FCM (Flow Cytometry)
1/200-1/400
ELISA
1/10000
References
1,Int J Mol Sci. 2019 Mar 6;20(5):1137;2,J BUON. Mar-Apr 2019;24(2):770-778.
Product Image
ELISA
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
WESTERN BLOT
Figure 2: Western blot analysis using CD99 mAb against human CD99 (AA: 1-185) recombinant protein. (Expected MW is 43 kDa)
WESTERN BLOT
Figure 3: Western blot analysis using CD99 mAb against HEK293-6E (1) and CD99 (AA:1-185)-hIgGFc transfected HEK293-6E (2) cell lysate.
WESTERN BLOT
Figure 4: Western blot analysis using CD99 mouse mAb against THP-1 (1), U937 (2),MOLT4 (3), and L1210 (4) cell lysate.
FLOW CYTOMETRY
Figure 5: Flow cytometric analysis of Jurkat cells using CD99 mouse mAb (green) and negative control (red).
IMMUNOHISTOCHEMISTRY
Figure 6: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CD99 mouse mAb with DAB staining.
IMMUNOHISTOCHEMISTRY
Figure 7: Immunohistochemical analysis of paraffin-embedded ewing sarcoma tissues using CD99 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.