CD8a Primary Antibody
Item Information
Catalog #
Size
Price
Description
| The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. |
Product Overview
Entrez GenelD
925
Aliases
CD8; p32; Leu2
Clone#
7H4B10
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human CD8a (AA: 22–235) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Blood. 2015 Sep 17;126(12):1443-51. 2.Cytometry B Clin Cytom. 2012 May;82(3):145-50.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD8a mAb against human CD8a (AA: 22–235) recombinant protein. (Expected MW is 26.4 kDa)
Flow cytometric
Figure 4:Flow cytometric analysis of Jurkat cells using CD8a mouse mAb (green) and negative control (red).
Western Blot
Figure 4:Western blot analysis using CD8a mAb against HEK293 (1) and CD8a (AA: 22–235)-hIgGFc transfected HEK293 (2) cell lysate.
For Research Use Only. Not for use in diagnostic procedures.