CD8a Primary Antibody

Item Information
Catalog # Size/Concentration Price
31798 100ug $341.00
Specification
AliasesCD8; p32; Leu2
Clone#7H4B10
Entrez GeneID925
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeMouse IgG1
ImmunogenPurified recombinant fragment of human CD8a (AA: 22–235) expressed in E. Coli.
MW25.7kDa
Application
ELISA1/10000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
22–235
Catalog
31798
$341.00
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Images

Elisa

Cell Culture Products

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Cell Culture Products

Figure 2:Western blot analysis using CD8a mAb against human CD8a (AA: 22–235) recombinant protein. (Expected MW is 26.4 kDa)

Flow cytometric

Cell Culture Products

Figure 4:Flow cytometric analysis of Jurkat cells using CD8a mouse mAb (green) and negative control (red).

Western Blot

Cell Culture Products

Figure 4:Western blot analysis using CD8a mAb against HEK293 (1) and CD8a (AA: 22–235)-hIgGFc transfected HEK293 (2) cell lysate.

Product Overview
Description
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene.
References (references)
References (references) 1.Blood. 2015 Sep 17;126(12):1443-51. 2.Cytometry B Clin Cytom. 2012 May;82(3):145-50.

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