Mouse Monoclonal Antibody to CD85K
This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. The receptor can also function in antigen capture and presentation. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/c2c313daaca7cd130f107b45277154d009cd223df4df69b3633d373c0020e5d4.jpg?v=1666705350)
![Figure 2:Western blot analysis using CD85K mAb against human CD85K (AA: 22-259) recombinant protein. (Expected MW is 52.2 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/2b360bbfdac61f276a8c057ef788522a71a78378153a2142726dde4a04bf508a.jpg?v=1666705350)
![Figure 3:Western blot analysis using CD85K mAb against HEK293-6e (1) and CD85K (AA: 22-259)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/c0b02e831872843e3bb989e5988ff9d19974f48d7192695d4e34674bae771cbd.jpg?v=1666705350)
![Figure 4:Flow cytometric analysis of HL-60 cells using CD85K mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/965b5c9c4ae84147c20609afcecea266834a6cc5e4f58d6955ed4940c5350e12.jpg?v=1666705350)
![Figure 5:Flow cytometric analysis of Jurkat cells using CD85K mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/058c02c426b0aacf58063208438220753f1c90830b517447d388d5d79ddd4a09.jpg?v=1666705350)
![Figure 6:Flow cytometric analysis of THP-1 cells using CD85K mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/48b6eb42db32af143586556ae7055684634f785053fa2578abf2cb248d2f66b2.jpg?v=1666705350)
![Figure 7:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using CD85K mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/a15dc79d631b4a92b0e2e5ab67bf4ec85ea4331a176101eada469c61485de74a.jpg?v=1666705350)
![Figure 8:Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using CD85K mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/efd5f6d2d399db081858182cffe9292da41e5ae6b9acb4c4d5032adaabef9f89.jpg?v=1666705350)
![Figure 9:Immunofluorescence analysis of Hela cells using CD85K mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/d9efe28e96e0a91e55a687c04c22ed2372660b69bc08673f6336a52db44be382.jpg?v=1666705350)