CD79B

Item Information

Catalog #

Size

Price

Description

The B lymphocyte antigen receptor is a multimeric complex that includes the antigen-specific component, surface immunoglobulin (Ig). Surface Ig non-covalently associates with two other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. This gene encodes the Ig-beta protein of the B-cell antigen component. Alternatively spliced transcript variants encoding different isoforms have been described.

Product Overview

Entrez GenelD

974

Aliases

B29; IGB; AGM6

Clone#

3H2H10

Host / Isotype

Mouse / Mouse IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human CD79B (AA:extra 30-160) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4℃; -20℃ for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/25 - 1/50

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.Eur J Haematol. 2019 Feb;102(2):191-196.
2.Leuk Lymphoma. 2016;57(6):1413-22.

Product Image

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using CD79B mAb against human CD79B (AA: extra 30-160) recombinant protein. (Expected MW is 41.1 kDa)

Western Blot

Figure 3:Western blot analysis using CD79B mAb against HEK293-6e (1) and CD79B (AA: extra 30-160)-hIgGFc transfected HEK293-6e (2) cell lysate.

Immunofluorescence analysis

Figure 4:Flow cytometric analysis of Raji cells using CD79B mouse mAb (green) and negative control (red).

Immunohistochemical analysis

Figure 5:Immunofluorescence analysis of Hela cells using CD79B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Secondary antibody from Fisher (Cat#: 35503)

Immunohistochemical analysis

Figure 6:Immunofluorescence analysis of Hela cells using CD79B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

For Research Use Only. Not for use in diagnostic procedures.