CD79B Primary Antibody
Item Information
Catalog #
Size
Price
Description
The B lymphocyte antigen receptor is a multimeric complex that includes the antigen-specific component, surface immunoglobulin (Ig). Surface Ig non-covalently associates with two other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. This gene encodes the Ig-beta protein of the B-cell antigen component. Alternatively spliced transcript variants encoding different isoforms have been described.
Product Overview
Entrez GenelD
974
Aliases
B29; IGB; AGM6
Clone#
3H2H10
Host / Isotype
Mouse / Mouse IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CD79B (AA:extra 30-160) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/25 - 1/50
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Eur J Haematol. 2019 Feb;102(2):191-196.
2.Leuk Lymphoma. 2016;57(6):1413-22.
2.Leuk Lymphoma. 2016;57(6):1413-22.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD79B mAb against human CD79B (AA: extra 30-160) recombinant protein. (Expected MW is 41.1 kDa)
Western Blot
Figure 3:Western blot analysis using CD79B mAb against HEK293-6e (1) and CD79B (AA: extra 30-160)-hIgGFc transfected HEK293-6e (2) cell lysate.
Immunofluorescence analysis
Figure 4:Flow cytometric analysis of Raji cells using CD79B mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5:Immunofluorescence analysis of Hela cells using CD79B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Secondary antibody from Fisher (Cat#: 35503)
Immunohistochemical analysis
Figure 6:Immunofluorescence analysis of Hela cells using CD79B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
For Research Use Only. Not for use in diagnostic procedures.