CD59 Primary Antibody
| Item Information | ||
|---|---|---|
| Catalog # | Size/Concentration | Price |
| Specification | |
|---|---|
| Aliases | 1F5; EJ16; EJ30; EL32; G344; MIN1; MIN2; MIN3; MIRL; HRF20; MACIF; MEM43; MIC11; MSK21; 16.3A5; HRF-20; MAC-IP; p18-20 |
| ProductOrder | C |
| Clone# | 8D2B8 |
| Entrez GenelD | 966 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | Purified recombinant fragment of human CD59 (AA: 31-111) expressed in E. Coli. |
| MW | 14.2kDa |
| Shipping Information | This product will ship in a box containing blue ice at a temperature of 4°C. Learn More |
| Species Reactivity | Human |
| Application | |
|---|---|
| ELISA | 1/10000 |
| ICC (Immunocytochemistry) | 1/50 - 1/200 |
| IHC_P(Immunohistochemistry) | 1/200 - 1/1000 |
| FCM (Flow Cytometry) | 1/200 - 1/400 |
| WB (Western Blot) | 1/500 - 1/2000 |
| Sequence | |
|---|---|
| 31-111 | |
Western Blot
Figure 1: Western blot analysis using CD59 mAb against human CD59 recombinant protein. (Expected MW is 34.7 kDa)
Western Blot
Figure 2: Western blot analysis using CD59 mAb against HEK293 (1) and CD59 (AA: 31-111)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of MCF-7 cells using CD59 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunofluorescence analysis
Figure 4: Immunofluorescence analysis of Hela cells using CD59 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric
Figure 5: Flow cytometric analysis of HeLa cells using CD59 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CD59 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using CD59 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);