CD44 Primary Antibody

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Description

CD44, also known as IN, LHRMIC4, CDW44, HCELL. It is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.

Product Overview
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
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Western Blot
Figure 1: Western blot analysis using CD44 mouse mAb against truncated Trx-CD44 recombinant protein (1) and GST-CD44 (aa628-699) recombinant protein (2).
Figure 1: Western blot analysis using CD44 mouse mAb against truncated Trx-CD44 recombinant protein (1) and GST-CD44 (aa628-699) recombinant protein (2).
Western Blot
Figure 2: Western blot analysis using CD44 mouse mAb against Hela (1) and HUVE-12(2) cell lysate.
Figure 2: Western blot analysis using CD44 mouse mAb against Hela (1) and HUVE-12(2) cell lysate.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissues, showing membrane localization with DAB staining using CD44 mouse mAb.
Figure 3: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissues, showing membrane localization with DAB staining using CD44 mouse mAb.
Immunofluorescence analysis
Figure 4: Confocal analysis of paraffin-embedded human lung cancer tissues using CD44 mouse mAb (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.
Figure 4: Confocal analysis of paraffin-embedded human lung cancer tissues using CD44 mouse mAb (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.
Immunofluorescence analysis
Figure 5: Confocal Immunofluorescence analysis of PANC-1 cells using CD44 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Figure 5: Confocal Immunofluorescence analysis of PANC-1 cells using CD44 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Immunofluorescence analysis
Figure 6: Confocal Immunofluorescence analysis of methanol-fixed A431 (A), Hela (B), PANC-1 (C) and EC (D) cells using CD44 mouse mAb (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.
Figure 6: Confocal Immunofluorescence analysis of methanol-fixed A431 (A), Hela (B), PANC-1 (C) and EC (D) cells using CD44 mouse mAb (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric
Figure 7: Flow cytometric analysis of Hela cells using CD44 mouse mAb (right) and negative control (left).
Figure 7: Flow cytometric analysis of Hela cells using CD44 mouse mAb (right) and negative control (left).
For Research Use Only. Not for use in diagnostic procedures.