CD43 Primary Antibody

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Description

This gene encodes a highly sialylated glycoprotein that functions in antigen-specific activation of T cells, and is found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It contains a mucin-like extracellular domain, a transmembrane region and a carboxy-terminal intracellular region. The extracellular domain has a high proportion of serine and threonine residues, allowing extensive O-glycosylation, and has one potential N-glycosylation site, while the carboxy-terminal region has potential phosphorylation sites that may mediate transduction of activation signals. Different glycoforms of this protein have been described. In stimulated immune cells, proteolytic cleavage of the extracellular domain occurs in some cell types, releasing a soluble extracellular fragment. Defects in expression of this gene are associated with Wiskott-Aldrich syndrome.

Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
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ELISA
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
WESTERN BLOT
Figure 2: Western blot analysis using CD43 mAb against human CD43(AA: extra(20-169)) recombinant protein. (Expected MW is 33.3kDa)
Figure 2: Western blot analysis using CD43 mAb against human CD43(AA: extra(20-169)) recombinant protein. (Expected MW is 33.3kDa)
WESTERN BLOT
Figure 3: Western blot analysis using CD43 mouse mAb against HEK293-6E (1), HEK293 (2),K562 (3), MOLT4 (4), Jurkat (5),and PANC-1 (6) cell lysate.
Figure 3: Western blot analysis using CD43 mouse mAb against HEK293-6E (1), HEK293 (2),K562 (3), MOLT4 (4), Jurkat (5),and PANC-1 (6) cell lysate.
FLOW CYTOMETRY
Figure 4: Flow cytometric analysis of Jurkat cells using CD43 mouse mAb (green) and negative control (red).
Figure 4: Flow cytometric analysis of Jurkat cells using CD43 mouse mAb (green) and negative control (red).
IMMUNOHISTOCHEMISTRY
Figure 5: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CD43 mouse mAb with DAB staining.
Figure 5: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CD43 mouse mAb with DAB staining.
WESTERN BLOT
Figure 6: Western blot analysis using CD43 mAb against HEK293-6E (1) and CD43(AA: extra(20-169))-hIgGFc transfected HEK293-6E (2) cell lysate.
Figure 6: Western blot analysis using CD43 mAb against HEK293-6E (1) and CD43(AA: extra(20-169))-hIgGFc transfected HEK293-6E (2) cell lysate.
For Research Use Only. Not for use in diagnostic procedures.