CD43 Primary Antibody

Item Information
Catalog #
Size
Price
Description

This gene encodes a highly sialylated glycoprotein that functions in antigen-specific activation of T cells, and is found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It contains a mucin-like extracellular domain, a transmembrane region and a carboxy-terminal intracellular region. The extracellular domain has a high proportion of serine and threonine residues, allowing extensive O-glycosylation, and has one potential N-glycosylation site, while the carboxy-terminal region has potential phosphorylation sites that may mediate transduction of activation signals. Different glycoforms of this protein have been described. In stimulated immune cells, proteolytic cleavage of the extracellular domain occurs in some cell types, releasing a soluble extracellular fragment. Defects in expression of this gene are associated with Wiskott-Aldrich syndrome.

Product Overview
Entrez GenelD
6693
Aliases
SPN; LSN; GALGP; GPL115
Clone#
1H8B10
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CD43 (AA: extra 20-253) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Ann Diagn Pathol. 2017 Aug;29:23-27.
2.Hum Pathol. 2015 Apr;46(4):593-9.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD43 mAb against human CD43 (AA: extra 20-253) recombinant protein. (Expected MW is 49.4 kDa)
Figure 2:Western blot analysis using CD43 mAb against human CD43 (AA: extra 20-253) recombinant protein. (Expected MW is 49.4 kDa)
Western Blot
Figure 3:Western blot analysis using CD43 mAb against HEK293 (1) and CD43 (AA: extra 20-253)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using CD43 mAb against HEK293 (1) and CD43 (AA: extra 20-253)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of HL-60 cells using CD43 mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of HL-60 cells using CD43 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.