CD40 Primary Antibody

Item Information
Catalog # Size/Concentration Price
32002 100ug $358.00
Specification
Aliasesp50; Bp50; CDW40; TNFRSF5
Clone#4E4C6
Entrez GeneID958
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeMouse IgG1
ImmunogenPurified recombinant fragment of human CD40 (AA: extra(21-193)) expressed in E. Coli.
MW30.6kDa
Application
ELISA1/10000
IHC_P (Immunohistochemistry) 1/200-1/1000
FCM (Flow Cytometry)1/200-1/400
WB (Western Blot)1/500 - 1/2000
Sequence
AA: 21-193
Catalog
32002
$358.00
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Images

ELISA

Cell Culture Products
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

WESTERN BLOT

Cell Culture Products
Figure 2: Western blot analysis using CD40 mAb against human CD40 (AA: extra(21-193)) recombinant protein. (Expected MW is 22 kDa)

WESTERN BLOT

Cell Culture Products
Figure 3: Western blot analysis using CD40 mAb against HEK293-6e (1) and CD40 (AA: extra(21-193))-hIgGFc transfected HEK293-6e (2) cell lysate.

WESTERN BLOT

Cell Culture Products
Figure 4: Western blot analysis using CD40 mouse mAb against Raji cell lysate.

FLOW CYTOMETRY

Cell Culture Products
Figure 5: Flow cytometric analysis of Raji cells using CD40 mouse mAb (green) and negative control (red).

IMMUNOHISTOCHEMISTRY

Cell Culture Products
Figure 6: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CD40 mouse mAb with DAB staining.

IMMUNOHISTOCHEMISTRY

Cell Culture Products
Figure 7: Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using CD40 mouse mAb with DAB staining.
Product Overview
Description

This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2014]

References (references)
References (references) 1,Life Sci. 2020 Aug 1;254:117774. 2,Mol Genet Genomic Med. 2019 Dec;7(12):e1014.

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