Mouse Monoclonal Antibody to CD4
This gene encodes the CD4 membrane glycoprotein of T lymphocytes. The CD4 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class II MHC molecules. The CD4 antigen is also a primary receptor for entry of the human immunodeficiency virus through interactions with the HIV Env gp120 subunit. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, granulocytes, as well as in various regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene.
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/925e701e2381fa777741a752c8bf34caba7a8b5cd46262c501e308cbb774f24c.jpg?v=1666706093)
![Figure 2:Western blot analysis using CD4 mAb against human CD4 (AA: 26-170) recombinant protein. (Expected MW is 42kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/bd2262441614962e4fe3fcd2c75505064c31ec48b294797b550c3354b8c2b39c.jpg?v=1666706093)
![Figure 3:Western blot analysis using CD4 mAb against HEK293-6e (1) and CD4 (AA: 26-170)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/f9f9f449154b46a7f85ebc1f31b1f008b3c82e0c20cc6a2c902d950eae1a7372.jpg?v=1666706093)
![Figure 4:Flow cytometric analysis of Jurkat cells using CD4 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/a9330d441e46ca07de502d53ab86f27950f310a9651b5f99fc77d3bf492cd510.jpg?v=1666706093)