Mouse Monoclonal Antibody to CD4

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesIMD79; OKT4D; CD4mut
Clone#3A12A5
Entrez GenelD920
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG2b
ImmunogenPurified recombinant fragment of human CD4 (AA: 26-170) expressed in E. Coli.
MW51kDa
Application
ELISA1/10000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
26-170
Catalog#: 32256
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Images
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD4 mAb against human CD4 (AA: 26-170) recombinant protein. (Expected MW is 42kDa)
Figure 2:Western blot analysis using CD4 mAb against human CD4 (AA: 26-170) recombinant protein. (Expected MW is 42kDa)
Western Blot
Figure 3:Western blot analysis using CD4 mAb against HEK293-6e (1) and CD4 (AA: 26-170)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using CD4 mAb against HEK293-6e (1) and CD4 (AA: 26-170)-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
Figure 4:Flow cytometric analysis of Jurkat cells using CD4 mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of Jurkat cells using CD4 mouse mAb (green) and negative control (red).
Product Overview
Description

This gene encodes the CD4 membrane glycoprotein of T lymphocytes. The CD4 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class II MHC molecules. The CD4 antigen is also a primary receptor for entry of the human immunodeficiency virus through interactions with the HIV Env gp120 subunit. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, granulocytes, as well as in various regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene.

References (references)
References (references)1.Biochem Biophys Res Commun. 2018 Mar 4;497(2):811-817.2.Eur J Immunol. 2017 Jul;47(7):1232-1242.