CD3G Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is the CD3-gamma polypeptide, which together with CD3-epsilon, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. Defects in this gene are associated with T cell immunodeficiency.
Product Overview
Entrez GenelD
917
Aliases
T3G; IMD17; CD3-GAMMA
Clone#
5B7B2
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CD3G (AA: extra 23-116) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Scand J Immunol. 2014 Nov;80(5):354-61.
2.DNA Cell Biol. 2012 Sep;31(9):1480-5.
2.DNA Cell Biol. 2012 Sep;31(9):1480-5.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD3G mAb against human CD3G (AA: extra 23-116) recombinant protein. (Expected MW is 36.6 kDa)
Western Blot
Figure 3:Western blot analysis using CD3G mAb against HEK293 (1) and CD3G (AA: extra 23-116)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using CD3G mouse mAb against Jurkat (1) cell lysate.
Flow cytometric
Figure 5:Flow cytometric analysis of Raji cells using CD3G mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.