CD3D Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined.
Product Overview
Entrez GenelD
915
Aliases
T3D; IMD19; CD3-DELTA
Clone#
1F6A8
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CD3D (AA: extra 22-105) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.J Clin Invest. 2011 Oct;121(10):3872-6.
2.Cytometry B Clin Cytom. 2009 May;76(3):213-7.
2.Cytometry B Clin Cytom. 2009 May;76(3):213-7.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD3D mAb against human CD3D (AA: extra 22-105) recombinant protein. (Expected MW is 35.5 kDa)
Western Blot
Figure 3:Western blot analysis using CD3D mAb against HEK293 (1) and CD3D (AA: extra 22-105)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of HL-60 cells using CD3D mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.