CD31 Primary Antibody

Item Information

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Price

Description

CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors.It is constitutively expressed on the surface of endothelial cells, and concentrated at the junction between them. The antibody reacts with the murine form of the Platelet-Endothelial Cell Adhesion Molecule. The reactivity of the antibody is restricted to the isoform of the molecule that is selectively expressed by endothelial cells.The antigen is predominantly present at the lateral borders of endothelial cells as described for human PECAM-1.It is also weakly expressed on many peripheral lymphoid cells and platelets.CD31 has been used to measure angiogenesis in association with tumor recurrence. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas).

Product Overview

Entrez GenelD

5175

Aliases

CD31; PECAM-1; PECAM1

Clone#

2F7B2

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human CD31 expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1. Mayr U et al. Circ Res 98:412-20 (2006).
2. Bingle L et al. Br J Cancer 94:101-7 (2006).
3. Wynne F et al. Reproduction 131:721-32 (2006).

Product Image

Western Blot

Figure 1: Western blot analysis using CD31 mouse mAb against truncated CD31 recombinant protein.

Immunohistochemical analysis

Figure 2: Immunohistochemical analysis of paraffin-embedded human lung cancer (A), lymphonodus tissue (B) and breast cancer (C), showing cytoplasmic localization of vascular endothelial cells using CD31 mouse mAb with DAB staining.

Immunohistochemical analysis

Figure 3: Immunohistochemical analysis of paraffin-embedded human placenta using CD31 mouse mAb.

Immunofluorescence analysis

Figure 4:Immunofluorescence analysis of paraffin-embedded human lung cancer(left) and breast cancer(right) cells using CD31 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

For Research Use Only. Not for use in diagnostic procedures.