CD275 Primary Antibody
Item Information
Catalog #
Size
Price
Description
Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function (By similarity).
Product Overview
Entrez GenelD
23308
Aliases
ICOSLG; B7H2; GL50; B7-H2; B7RP1; ICOSL; LICOS; B7RP-1; ICOS-L
Clone#
1G6E1
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CD275 (AA: extra 19-256) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Immunity. 2014 May 15;40(5):734-46.
2.Cancer Res. 2012 Dec 1;72(23):6130-41.
2.Cancer Res. 2012 Dec 1;72(23):6130-41.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD275 mAb against human CD275 (AA: extra 19-256) recombinant protein. (Expected MW is 52.4 kDa)
Western Blot
Figure 3:Western blot analysis using CD275 mAb against HEK293 (1) and CD275 (AA: extra 19-256)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of HL-60 cells using CD275 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.