CD274 Primary Antibody

Item Information
Catalog #
Size
Price
Description
This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants.
Product Overview
Entrez GenelD
29126
Aliases
B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
Clone#
7D2A10
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CD274 (AA: 24-153) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Anticancer Res. 2015 Oct;35(10):5369-76.
2.Lung Cancer. 2015 Jun;88(3):254-9.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD274 mAb against human CD274 (AA: 24-153) recombinant protein. (Expected MW is 40.1 kDa)
Figure 2:Western blot analysis using CD274 mAb against human CD274 (AA: 24-153) recombinant protein. (Expected MW is 40.1 kDa)
Western Blot
Figure 3:Western blot analysis using *** mAb against HEK293 (1) and *** (AA: ***)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using *** mAb against HEK293 (1) and *** (AA: ***)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of Hela cells using CD274 mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of Hela cells using CD274 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.