CD235a

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesMN; GPA; MNS; GPSAT; PAS-2; GPErik; HGpMiV; HGpMiXI; HGpSta(C)
Clone#2D5A6
Entrez GenelD2993
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeMouse IgG1
ImmunogenPurified recombinant fragment of human CD235a (AA: 20-91) expressed in E. Coli.
MW16.3kDa
Species ReactivityHuman
Application
ELISA1/10000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
extra(20-91)
Catalog#: 32284
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Images
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD235a mAb against human CD235a (AA: 20-91) recombinant protein. (Expected MW is 34 kDa)
Figure 2:Western blot analysis using CD235a mAb against human CD235a (AA: 20-91) recombinant protein. (Expected MW is 34 kDa)
Western Blot
Figure 3:Western blot analysis using CD235a mAb against HEK293-6e (1) and CD235a (AA: 20-91)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using CD235a mAb against HEK293-6e (1) and CD235a (AA: 20-91)-hIgGFc transfected HEK293-6e (2) cell lysate.
Immunofluorescence analysis
Figure 4:Flow cytometric analysis of K562 cells using CD235a mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of K562 cells using CD235a mouse mAb (green) and negative control (red).
Product Overview
Description

Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. In addition to the M or N and S or s antigens that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta, as well as Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are the result of gene recombinations between GYPA and GYPB.

References (references)
References (references)1.Transfusion. 2020 Jun;60(6):1287-1293.
2.Hum Genet. 2018 Feb;137(2):151-160.