CD22 Primary Antibody

Item Information
Catalog # Size/Concentration Price
30549 100μl $341.00
Product OrderC
Entrez GeneID933
FormulationPurified antibody in PBS with 0.05% sodium azide
ImmunogenPurified recombinant fragment of human CD22 (AA: 621-725) expressed in E. Coli.
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C.  Learn More
Species ReactivityHuman
ICC (Immunocytochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000

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Western Blot

Cell Culture Products
Figure 1: Western blot analysis using CD22 mAb against human CD22 recombinant protein. (Expected MW is 37 kDa)

Western Blot

Cell Culture Products
Figure 2: Western blot analysis using CD22 mAb against HEK293 (1) and CD22 (AA: 621-725)-hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis

Cell Culture Products
Figure 3: Immunofluorescence analysis of Hela cells using CD22 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow cytometric

Cell Culture Products
Figure 4: Flow cytometric analysis of Hela cells using CD22 mouse mAb (green) and negative control (red).


Cell Culture Products
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description CD22 may be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules
References (references)
References (references) 1. Cancer Res. 2012 Nov 1;72(21):5556-65.
2. J Innate Immun. 2011;3(4):411-9.

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