CD166 Primary Antibody
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Description
This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found.
Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
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Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD166 mAb against human CD166 (AA: extra 227-381) recombinant protein. (Expected MW is 47 kDa)
Western Blot
Figure 3:Western blot analysis using CD166 mAb against HEK293 (1) and CD166 (AA: extra 227-381)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of HL-60 cells using CD166 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CD166 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CD166 mouse mAb with DAB staining.
Flow cytometric
Figure 7:Flow cytometric analysis of K562 cells using CD166 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.