CD166 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found.
Product Overview
Entrez GenelD
214
Aliases
MEMD; ALCAM
Clone#
2F1B12
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CD166 (AA: extra 227-381) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Diagn Pathol. 2015 Jul 2;10:86.
2.Asian Pac J Cancer Prev. 2015;16(9):3849-56.
2.Asian Pac J Cancer Prev. 2015;16(9):3849-56.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD166 mAb against human CD166 (AA: extra 227-381) recombinant protein. (Expected MW is 47 kDa)
Western Blot
Figure 3:Western blot analysis using CD166 mAb against HEK293 (1) and CD166 (AA: extra 227-381)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of HL-60 cells using CD166 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using CD166 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using CD166 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.