CD162

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesCLA; SELPLG; PSGL1; PSGL-1
Clone#3D5E11
Entrez GenelD6404
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeMouse IgG1
ImmunogenPurified recombinant fragment of human CD162 (AA: 42-320) expressed in E. Coli.
MW43.2kDa
Species ReactivityHuman
Application
ELISA1/10000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
extra(42-320)
Catalog#: 32295
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Images
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD162 mAb against human CD162 (AA: 42-320) recombinant protein. (Expected MW is 54.7 kDa)
Figure 2:Western blot analysis using CD162 mAb against human CD162 (AA: 42-320) recombinant protein. (Expected MW is 54.7 kDa)
Immunohistochemical analysis
Figure 4:Immunohistochemical analysis of paraffin-embedded cerebellum tissues using CD162 mouse mAb with DAB staining.
Figure 4:Immunohistochemical analysis of paraffin-embedded cerebellum tissues using CD162 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using CD162 mouse mAb with DAB staining.
Figure 5:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using CD162 mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 5:Flow cytometric analysis of THP-1 cells using CD162 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of THP-1 cells using CD162 mouse mAb (green) and negative control (red).
Product Overview
Description

This gene encodes a glycoprotein that functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Alternate splicing results in multiple transcript variants.

References (references)
References (references)1.World J Gastroenterol. 2020 Nov 7;26(41):6361-6377.
2.Nat Microbiol. 2019 May;4(5):813-825.