CD156B

Item Information
Catalog #
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Price
Description

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The encoded preproprotein is proteolytically processed to generate the mature protease. The encoded protease functions in the ectodomain shedding of tumor necrosis factor-alpha, in which soluble tumor necrosis factor-alpha is released from the membrane-bound precursor. This protease also functions in the processing of numerous other substrates, including cell adhesion proteins, cytokine and growth factor receptors and epidermal growth factor (EGF) receptor ligands, and plays a prominent role in the activation of the Notch signaling pathway. Elevated expression of this gene has been observed in specific cell types derived from psoriasis, rheumatoid arthritis, multiple sclerosis and Crohn's disease patients, suggesting that the encoded protein may play a role in autoimmune disease. Additionally, this protease may play a role in viral infection through its cleavage of ACE2, the cellular receptor for SARS-CoV and SARS-CoV-2.

Product Overview
Entrez GenelD
6868
Aliases
ADAM17;CSVP; TACE; NISBD; ADAM18;; NISBD1
Clone#
7A6B2
Host / Isotype
Mouse / Mouse IgG1
Species Reactivity
Human, Mouse
Immunogen
Purified recombinant fragment of human CD156B (AA: 497-671) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Nephrol Dial Transplant. 2020 Jun 1;35(6):1071-1072.
2.Eur J Clin Pharmacol. 2020 Dec;76(12):1623-1630.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD156B mAb against human CD156B (AA: 497-671) recombinant protein. (Expected MW is 45.3 kDa)
Figure 2:Western blot analysis using CD156B mAb against human CD156B (AA: 497-671) recombinant protein. (Expected MW is 45.3 kDa)
Western Blot
Figure 3:Western blot analysis using CD156B mAb against HEK293-6e (1) and CD156B (AA: 497-671)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using CD156B mAb against HEK293-6e (1) and CD156B (AA: 497-671)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using CD156B mouse mAb against Jurkat (1) cell lysate.
Figure 4:Western blot analysis using CD156B mouse mAb against Jurkat (1) cell lysate.
Immunofluorescence analysis
Figure 5:Flow cytometric analysis of Hela cells using CD156B mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of Hela cells using CD156B mouse mAb (green) and negative control (red).
Immunofluorescence analysis
Figure 6:Flow cytometric analysis of NIH/3T3 cells using CD156B mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of NIH/3T3 cells using CD156B mouse mAb (green) and negative control (red).
Immunofluorescence analysis
Figure 7:Flow cytometric analysis of Raji cells using CD156B mouse mAb (green) and negative control (red).
Figure 7:Flow cytometric analysis of Raji cells using CD156B mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 8:Immunofluorescence analysis of Hela cells using CD156B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.Secondary antibody from Fisher (Cat#: 35503)
Figure 8:Immunofluorescence analysis of Hela cells using CD156B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.Secondary antibody from Fisher (Cat#: 35503)
Immunohistochemical analysis
Figure 9:Immunofluorescence analysis of Hela cells using CD156B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 9:Immunofluorescence analysis of Hela cells using CD156B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
For Research Use Only. Not for use in diagnostic procedures.