CD154 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.
Product Overview
Entrez GenelD
959
Aliases
CD40LG; IGM; IMD3; TRAP; gp39; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L
Clone#
5A3A9
Host / Isotype
Mouse / IgG2a
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CD154 (AA: extra 47-261) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Hematology. 2016 Apr;21(3):187-92.
2.Mol Cell Probes. 2015 Dec;29(6):335-342.
2.Mol Cell Probes. 2015 Dec;29(6):335-342.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD154 mAb against human CD154 (AA: extra 47-261) recombinant protein. (Expected MW is 50 kDa)
Western Blot
Figure 3:Western blot analysis using CD154 mAb against HEK293 (1) and CD154 (AA: extra 47-261)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of Ramos cells using CD154 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.