CD147 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. The encoded protein is also a member of the immunoglobulin superfamily. Multiple transcript variants encoding different isoforms have been found for this gene.
Product Overview
Entrez GenelD
682
Aliases
BSG; OK; 5F7; TCSF; EMMPRIN
Clone#
2D8C11
Host / Isotype
Mouse / Mouse IgG2b
Immunogen
Purified recombinant fragment of human CD147 (AA: extra 138-323) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200-1/1000
FCM (Flow Cytometry)
1/200-1/400
ELISA
1/10000
References
1.Oncotarget. 2016 Feb 2;7(5):5613-29. 2.J Biochem. 2016 May;159(5):481-90.
Product Image
ELISA
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
WESTERN BLOT
Figure 2: Western blot analysis using CD147 mAb against human CD147 (AA: extra 138-323) recombinant protein. (Expected MW is 46.3 kDa)
WESTERN BLOT
Figure 3: Western blot analysis using CD147 mAb against HEK293-6e (1) and CD147 (AA: extra 138-323)-hIgGFc transfected HEK293-6e (2) cell lysate.
FLOW CYTOMETRY
Figure 4: Flow cytometric analysis of Hela cells using CD147 mouse mAb (green) and negative control (red).
IMMUNOHISTOCHEMISTRY
Figure 5: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using CD147 mouse mAb with DAB staining.
IMMUNOHISTOCHEMISTRY
Figure 6: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using CD147 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.