CD125 Primary Antibody

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Description

The protein encoded by this gene is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4. Several alternatively spliced transcript variants encoding four distinct isoforms have been reported.

Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD125 mAb against human CD125 (AA: extra 21-196) recombinant protein. (Expected MW is 45.9 kDa)
Figure 2:Western blot analysis using CD125 mAb against human CD125 (AA: extra 21-196) recombinant protein. (Expected MW is 45.9 kDa)
Western Blot
Figure 3:Western blot analysis using CD125 mAb against HEK293 (1) and CD125 (AA: extra 21-196)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using CD125 mAb against HEK293 (1) and CD125 (AA: extra 21-196)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of HL-60 cells using CD125 mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of HL-60 cells using CD125 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.