CD122 Primary Antibody
The interleukin 2 receptor, which is involved in T cell-mediated immune responses, is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. The protein encoded by this gene represents the beta subunit and is a type I membrane protein. The use of alternative promoters results in multiple transcript variants encoding the same protein. The protein is primarily expressed in the hematopoietic system. The use by some variants of an alternate promoter in an upstream long terminal repeat (LTR) results in placenta-specific expression.
Purified antibody in PBS with 0.05% sodium azide
4°C; -20°C for long term storage
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using CD122 mAb against human CD122 (AA: extra 27-240) recombinant protein. (Expected MW is 50.6 kDa)
Figure 3:Western blot analysis using CD122 mAb against HEK293 (1) and CD122 (AA: extra 27-240)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Flow cytometric analysis of Ramos cells using CD122 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.