CD106 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene.
Product Overview
Entrez GenelD
7412
Aliases
VCAM1; INCAM-100
Clone#
6A11D8
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Monkey, Rat
Immunogen
Purified recombinant fragment of human CD106 (AA: extra 25-183) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Stem Cell Res Ther. 2016 Apr 4;7:49.2.Cell Signal. 2015 Dec;27(12):2467-73.
Product Image
Elisa
![Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/31490ea.jpg?v=1666703781)
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using CD106 mAb against human CD106 (AA: extra 25-183) recombinant protein. (Expected MW is 43.6 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/31490rwb.jpg?v=1666703781)
Figure 2:Western blot analysis using CD106 mAb against human CD106 (AA: extra 25-183) recombinant protein. (Expected MW is 43.6 kDa)
Western Blot
![Figure 3:Western blot analysis using CD106 mAb against HEK293 (1) and CD106 (AA: extra 25-183)-hIgGFc transfected HEK293 (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/31490twb.jpg?v=1666703781)
Figure 3:Western blot analysis using CD106 mAb against HEK293 (1) and CD106 (AA: extra 25-183)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
![Figure 4:Western blot analysis using CD106 mouse mAb against EC (1), COS7 (2), MCF-7 (3), HepG2 (4), and Hela (5) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/31490lwb.jpg?v=1666703781)
Figure 4:Western blot analysis using CD106 mouse mAb against EC (1), COS7 (2), MCF-7 (3), HepG2 (4), and Hela (5) cell lysate.
Immunofluorescence analysis
![Figure 5:Immunofluorescence analysis of Hela cells using CD106 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/31490icc.jpg?v=1666703781)
Figure 5:Immunofluorescence analysis of Hela cells using CD106 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
![Figure 6:Flow cytometric analysis of HL-60 cells using CD106 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/31490fcm.jpg?v=1666703781)
Figure 6:Flow cytometric analysis of HL-60 cells using CD106 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.