CD106 Primary Antibody

Item Information

Catalog #

Size

Price

Description

This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene.

Product Overview

Entrez GenelD

7412

Aliases

VCAM1; INCAM-100

Clone#

6A11D8

Host / Isotype

Mouse / IgG1

Species Reactivity

Human, Monkey, Rat

Immunogen

Purified recombinant fragment of human CD106 (AA: extra 25-183) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.Stem Cell Res Ther. 2016 Apr 4;7:49.2.Cell Signal. 2015 Dec;27(12):2467-73.

Product Image

Elisa

Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using CD106 mAb against human CD106 (AA: extra 25-183) recombinant protein. (Expected MW is 43.6 kDa)

Western Blot

Figure 3:Western blot analysis using CD106 mAb against HEK293 (1) and CD106 (AA: extra 25-183)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using CD106 mouse mAb against EC (1), COS7 (2), MCF-7 (3), HepG2 (4), and Hela (5) cell lysate.

Immunofluorescence analysis

Figure 5:Immunofluorescence analysis of Hela cells using CD106 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric