CD10 Primary Antibody
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Description
This gene encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. This gene, which encodes a 100-kD type II transmembrane glycoprotein, exists in a single copy of greater than 45 kb. The 5' untranslated region of this gene is alternatively spliced, resulting in four separate mRNA transcripts. The coding region is not affected by alternative splicing.
Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Image
Elisa

Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot

Figure 2:Western blot analysis using CD10 mAb against human CD10 (AA: extra 549-750) recombinant protein. (Expected MW is 48.8 kDa)
Western Blot

Figure 3:Western blot analysis using CD10 mAb against HEK293 (1) and CD10 (AA: extra 549-750)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot

Figure 4:Western blot analysis using CD10 mouse mAb against LNcap (1), Ramos (2), Raji (3), and NTERA-2 (4) cell lysate.
Flow cytometric

Figure 5:Flow cytometric analysis of K562 cells using CD10 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.