CD10 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. This gene, which encodes a 100-kD type II transmembrane glycoprotein, exists in a single copy of greater than 45 kb. The 5' untranslated region of this gene is alternatively spliced, resulting in four separate mRNA transcripts. The coding region is not affected by alternative splicing.
Product Overview
Entrez GenelD
4311
Aliases
MME; NEP; SFE; CALLA
Clone#
7D4B1
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CD10 (AA: 52-246) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. Pathol Res Pract. 2012 May 15;208(5):281-5.
2. J Dermatol Sci. 2013 Feb;69(2):105-13.
2. J Dermatol Sci. 2013 Feb;69(2):105-13.
Product Image
Western Blot
Figure 1: Western blot analysis using CD10 mAb against human CD10 (AA: 52-246) recombinant protein. (Expected MW is 41.4 kDa)
Western Blot
Figure 2: Western blot analysis using CD10 mAb against HEK293 (1) and CD10 (AA: 52-246)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using CD10 mouse mAb against LNCAP cell lysate.
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using CD10 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CD10 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.