CD10 Primary Antibody

Item Information
Catalog #
Size
Price
Description

CD10(MME): membrane metallo-endopeptidase. This gene encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. This gene, which encodes a 100-kD type II transmembrane glycoprotein, exists in a single copy of greater than 45 kb. The 5' untranslated region of this gene is alternatively spliced, resulting in four separate mRNA transcripts. The coding region is not affected by alternative splicing.

Product Overview
Entrez GenelD
4311
Aliases
NEP; CALLA
Clone#
3G9D10
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of CD10 expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. Journal of Gastroenterology,1996 Feb.31(1):12-7.
2. British Journal of Haematology,1995,89(3):623-6.
Product Image
Western Blot
Figure 1: Western blot analysis using CD10 mouse mAb against truncated CD10-His recombinant protein (1).
Figure 1: Western blot analysis using CD10 mouse mAb against truncated CD10-His recombinant protein (1).
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded human breast ductal myoepithelium,showing cytoplasmic and membrane location with DAB staining using CD10 mouse mAb.
Figure 2: Immunohistochemical analysis of paraffin-embedded human breast ductal myoepithelium,showing cytoplasmic and membrane location with DAB staining using CD10 mouse mAb.
For Research Use Only. Not for use in diagnostic procedures.